Abcam’s I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
Belongs to the intercrine alpha (chemokine CxC) family.
Brenndörfer ED et al. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol192:1671-80 (2014).
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