製品の概要

  • 製品名Mouse I-TAC ELISA Kit (CXCL11)
    CXCL11 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    5.487 pg/ml - 4000 pg/ml
  • 添加回収試験

    80 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 74.46 67% - 86%
    Serum 91.12 80% - 101%
    Plasma 75.6 68% - 88%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • 組織特異性High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • b R1
    • Beta R1
    • Beta-R1
    • betaR1
    • C X C motif chemokine 11
    • C-X-C motif chemokine 11
    • Chemokine (C-X-C motif) ligand 11
    • Chemokine C-X-C motif ligand 11
    • CXCL11
    • CXL11_HUMAN
    • H174
    • I TAC
    • I-TAC
    • Interferon gamma inducible protein 9
    • Interferon gamma-inducible protein 9
    • Interferon inducible T cell a chemoattractant I TAC
    • Interferon inducible T cell alpha chemoattractant
    • Interferon-inducible T-cell alpha chemoattractant
    • IP 9
    • IP-9
    • IP9
    • ITAC
    • MGC102770
    • SCYB11
    • SCYB9B
    • Small inducible cytokine B11
    • Small inducible cytokine subfamily B (Cys X Cys) member 11
    • Small inducible cytokine subfamily B (Cys X Cys) member 9B
    • Small inducible cytokine subfamily B, member 11
    • Small inducible cytokine subfamily B, member 9B
    • Small-inducible cytokine B11
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse I-TAC ELISA Kit (CXCL11) 画像

  • Representative standard curve using ab100716

  • Representative standard curve using ab100716

プロトコール

Mouse I-TAC ELISA Kit (CXCL11) (ab100716) 使用論文

This product has been referenced in:
  • Brenndörfer ED  et al. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol 192:1671-80 (2014). Sandwich ELISA ; Mouse . Read more (PubMed: 24442435) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab100716.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"