Mouse HGF ELISA Kit (ab100686)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.614 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse HGF ELISA Kit
HGF キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.4 ng/ml -
検出範囲
0.614 ng/ml - 60 ng/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 111.8 98% - 123% Serum 111.4 98% - 129% Plasma 105.8 80% - 120% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse HGF 2 vials HGF Microplate (12 x 8 wells) 1 unit Recombinant Mouse HGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
関連疾患
Deafness, autosomal recessive, 39 -
配列類似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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別名
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
参照データベース
- Entrez Gene: 15234 Mouse
- SwissProt: Q08048 Mouse
- Unigene: 267078 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100686 は 2 報の論文で使用されています。
- Boldyreva MA et al. Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle. Int J Mol Sci 20:N/A (2019). PubMed: 31238604
- El Ouaamari A et al. Liver-derived systemic factors drive ß cell hyperplasia in insulin-resistant states. Cell Rep 3:401-10 (2013). PubMed: 23375376