製品の概要

  • 製品名Mouse HGF ELISA Kit
    HGF キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.4 ng/ml
  • 検出範囲
    0.614 ng/ml - 60 ng/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 111.8 98% - 123%
    Serum 111.4 98% - 129%
    Plasma 105.8 80% - 120%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 関連疾患Deafness, autosomal recessive, 39
  • 配列類似性Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 別名
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A; scatter factor)
    • Hepatocyte growth factor beta chain
    • Hepatocyte growth factor precursor
    • Hepatopoietin A
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • OTTHUMP00000161349
    • OTTHUMP00000206710
    • OTTHUMP00000206711
    • OTTHUMP00000206712
    • OTTHUMP00000206713
    • OTTHUMP00000206730
    • Scatter factor
    • SF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse HGF ELISA Kit 画像

  • Representative Standard Curve using ab100686

  • Representative Standard Curve using ab100686

プロトコール

Mouse HGF ELISA Kit (ab100686) 使用論文

ab100686 has not yet been referenced specifically in any publications.

Product Wall

The antibody pairs used in this kit recognize both the alpha and beta chains of mouse HGF

The antibody pair inab100686 recognizes an epitope located somewhere between aa 33-728 (which is whole sequence minus the signal peptide). Therefore the kit is expected to detect the pro (uncleaved) form, the heterodimeric form, the alpha subunit, and ...

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Thank you for contacting us.

Both mouse and human HGF ELISA kits will detect total HGF.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your calls this week and for your patience.

I've heard back from my contact at the lab, and the standard is biologically active. Activity was measured as the ability to stimulate H-thymidine incorporation in 4MBr-5 monkey cells....

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Thank you for contacting Abcam. Unfortunately, epitope mapping has not been performed for this kit so we do not know exactly where the antibodies are binding.  Therefore whether the kit will recognize HGF bound to the c-met receptor or inhibit c-met fr...

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The best kit to use for tissue samples is ab100687 HGF Mouse ELISA Kit - 1 x 96 Well Plate. This is specifically meant for tissue samples. Both of the antibodies in the kit are polyclonal and considering that the homology between mouse and rat is hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"