Mouse heart tissue lysate - mitochondrial extract (ab110350)

製品の概要

  • 製品名
    Mouse heart tissue lysate - mitochondrial extract
    Mouse Heart ライゼート 製品一覧
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

    2 mg of mouse heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • アプリケーション
    適用あり: SDS-PAGE, WBmore details

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファー
    Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • ライゼート 備考
    2 mg of mouse heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

    アプリケーション

    Our Abpromise guarantee covers the use of ab110350 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    SDS-PAGE Use at an assay dependent dilution.
    WB Use at an assay dependent dilution.

    Mouse heart tissue lysate - mitochondrial extract (ab110350) 使用論文

    ab110350 has not yet been referenced specifically in any publications.

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    This product description is somewhat misleading. It is a mitochondrial fraction from pooled mouse hearts: hearts are minced and outer membranes ruptured by dounce homogenization, mitochondria are isolated by differ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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