- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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AMPA selective glutamate receptor 1
AMPA-selective glutamate receptor 1
Glutamate receptor 1
Glutamate receptor ionotropic
Glutamate receptor ionotropic AMPA 1
Glutamate receptor, ionotropic, AMPA 1
機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
組織特異性Widely expressed in brain.
配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
翻訳後修飾Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Western blot - Mouse Glutamate Receptor 1 (AMPA subtype) (phospho T840) peptide (ab12399)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Performed under reducing conditions.
Exposure time : 5 minutes
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
ab12108 detects 2 bands over 100kDa by Western Blot. Both of these bands are blocked by the immunizing phospho peptide (lane 4), but not by the non-modified peptide (lane 5). However the immunoprecipitation data (below) demonstrates that the upper band (at ~120kDa) is unlikely to be GluR1 and therefore is an unknown protein.