製品の概要

  • 製品名Mouse Galectin 1 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    156 pg/ml - 10000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse Galectin 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Galectin 1 in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Galectin 1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Galectin 1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Galectin 1 amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • 組織特異性Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • 配列類似性Contains 1 galectin domain.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • 14 kDa laminin-binding protein
    • 14 kDa lectin
    • Beta galactoside binding protein 14kDa
    • Beta-galactoside-binding lectin L-14-I
    • Gal-1
    • GAL1
    • Galaptin
    • Galectin-1
    • GBP
    • HBL
    • HLBP14
    • HPL
    • Lactose-binding lectin 1
    • Lectin galactoside-binding soluble 1
    • LEG1_HUMAN
    • LGALS1
    • Putative MAPK-activating protein PM12
    • S-Lac lectin 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Galectin 1 ELISA Kit 画像

  • Representative Standard Curve using ab119595

プロトコール

Mouse Galectin 1 ELISA Kit (ab119595) 使用論文

ab119595 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Your credit note ID is ***

I am sorry that thiskit did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purch...

Read More

Thank you very much for contacting us and letting us know about this problem.

I am sorry that this kit did not work as it should have. I'm happy to offer you a free of charge replacement, credit, or refund. Which would you prefer?

C...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"