製品の概要

  • 製品名
    Mouse Galectin 1 ELISA Kit
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    156 pg/ml - 10000 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse Galectin 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Galectin 1 in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Galectin 1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Galectin 1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Galectin 1 amount of sample captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-mouse Galectin 1 antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-mouse Galectin-1 antibody 1 x 130µl
    Lyophilized recombinant mouse Galectin 1 standard 2 x 10ng
    Plate Seal 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • 研究分野
  • 機能
    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • 組織特異性
    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • 配列類似性
    Contains 1 galectin domain.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • 14 kDa laminin-binding protein
    • 14 kDa lectin
    • Beta galactoside binding protein 14kDa
    • Beta-galactoside-binding lectin L-14-I
    • Gal-1
    • GAL1
    • Galaptin
    • Galectin-1
    • GBP
    • HBL
    • HLBP14
    • HPL
    • Lactose-binding lectin 1
    • Lectin galactoside-binding soluble 1
    • LEG1_HUMAN
    • LGALS1
    • Putative MAPK-activating protein PM12
    • S-Lac lectin 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119595

プロトコール

参考文献

ab119595 has not yet been referenced specifically in any publications.

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