Mouse Galectin 1 ELISA Kit (ab119595)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Galectin 1 ELISA Kit
Galectin 1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 343pg/ml 22.29 = 6.5% 2 16 1747pg/ml 132.77 = 7.6% 3 16 4774pg/ml 300.76 = 8.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 318pg/ml 20.67 = 6.5% 2 24 1697pg/ml 144.24 = 8.5% 3 24 4684pg/ml 304.46 = 6.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5 pg/ml -
検出範囲
156 pg/ml - 10000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s mouse Galectin 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Galectin 1 in cell culture supernatants, serum and plasma (heparin, EDTA).
A Galectin 1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Galectin 1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Galectin 1 amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-mouse Galectin 1 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-mouse Galectin-1 antibody 1 x 100µl Lyophilized recombinant mouse Galectin 1 standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究分野
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機能
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
組織特異性
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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別名
- 14 kDa laminin-binding protein
- 14 kDa lectin
- Beta galactoside binding lectin
see all -
参照データベース
- Entrez Gene: 16852 Mouse
- SwissProt: P16045 Mouse
- Unigene: 43831 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab119595 は 3 報の論文で使用されています。
- Losada-Barragán M et al. Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection. Front Cell Infect Microbiol 9:252 (2019). PubMed: 31355153
- Hartman ES et al. Increased Reactive Oxygen Species and Cell Cycle Defects Contribute to Anemia in the RASA3 Mutant Mouse Model scat. Front Physiol 9:689 (2018). PubMed: 29922180
- Kanda A et al. Advanced glycation endproducts link inflammatory cues to upregulation of galectin-1 in diabetic retinopathy. Sci Rep 7:16168 (2017). Functional Studies ; Mouse . PubMed: 29170525