製品の概要

  • 製品名
    Mouse FGF basic ELISA Kit
    FGF basic キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Tissue Lysate
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    18.8 pg/ml - 600 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 119.7 105% - 129%
    Serum 84.63 78% - 100%
    Plasma 83.26 76% - 99%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s FGF basic Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse FGF basic in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Mouse FGF measured in biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 5-10 fold.

  • Mouse FGF standard curve: mean of duplicates (+/- SD), performed in Diluent A

  • Representative Standard Curve using ab100670.

  • Representative Standard Curve using ab100670.

プロトコール

参考文献

This product has been referenced in:
  • Yuan Y  et al. Photodynamic antimicrobial chemotherapy with the novel amino acid-porphyrin conjugate 4I: In vitro and in vivo studies. PLoS One 12:e0176529 (2017). ELISA ; Mouse . Read more (PubMed: 28493985) »
  • Murillo MM  et al. RAS interaction with PI3K p110a is required for tumor-induced angiogenesis. J Clin Invest 124:3601-11 (2014). Read more (PubMed: 25003191) »

See all 3 Publications for this product

レビューと Q&A

Thank you for your email.

I assume you are looking for a lysis buffer because you wish to test cell or tissue lysates samples with the kit? If so, please note that the ab100670 kit has not been validated with lysate samples, only serum, plasm...

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Thank you for contacting us.

Although this kit was neither designed nor tested with cell or tissue lysate samples, this kit as well as our other sandwich ELISA kits are very accommodating to many different sample types so lysates samples shou...

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Thank you for contacting us. We do not currently sell any ELISA kits that are specifically designed for use on rat samples. After checking with the lab, our mouse and human ELISA kits for these targets will be unlikely to cross-react with rat, but have...

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Thank you very much for contacting us.

Unfortunately, epitope mapping of the two antibody pairs has not been performed, so we do not know the exact amino acid sequence recognised and if these regions are specific for human and mouse, respect...

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Thank you for your phone call today. I have heard back from my contact at the laboratory, and I was told that the ELISA plates come pre-blocked so a blocking step is not necessary.  I hope this information is helpful, but please let me know if y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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