製品の概要

  • 製品名Mouse FAK Matched Antibody Pair Kit
  • 検出方法Colorimetric
  • アッセイタイプELISA set
  • 検出範囲
    31.2 pg/ml - 2000 pg/ml
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse FAK.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    This kit contains sufficient materials to perform ten 96-well ELISAs if using the assay procedure described in the booklet.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • アプリケーション適用あり: ELISAmore details
  • 試験プラットフォームReagents

製品の特性

  • 機能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 組織特異性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • ドメインThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻訳後修飾Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 細胞内局在Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 別名
    • FADK
    • FADK 1
    • FAK related non kinase polypeptide
    • FAK1
    • FAK1_HUMAN
    • Focal adhesion Kinase
    • Focal adhesion kinase 1
    • Focal adhesion kinase isoform FAK Del33
    • Focal adhesion kinase related nonkinase
    • FRNK
    • p125FAK
    • pp125FAK
    • PPP1R71
    • Protein phosphatase 1 regulatory subunit 71
    • Protein tyrosine kinase 2
    • Protein-tyrosine kinase 2
    • Ptk2
    • PTK2 protein tyrosine kinase 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab215072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Mouse FAK Matched Antibody Pair Kit 画像

  • Standard calibration curve. Background subtracted values are graphed.

プロトコール

Mouse FAK Matched Antibody Pair Kit (ab215072) 使用論文

ab215072 has not yet been referenced specifically in any publications.

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