製品の概要

  • 製品名
    Mouse FAK Matched Antibody Pair Kit
  • 検出方法
    Colorimetric
  • アッセイタイプ
    ELISA set
  • 検出範囲
    31.2 pg/ml - 2000 pg/ml
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse FAK. Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • アプリケーション
    適用あり: ELISAmore details
  • 試験プラットフォーム
    Reagents

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 10 x 96 tests 2 x 96 tests
    Mouse FAK Capture Antibody 1 x 100µg 1 x 20µg
    Mouse FAK Detector Antibody 1 x 25µg 1 x 5µg
    Mouse FAK Lyophilized Protein 1 vial 1 vial
  • 研究分野
  • 機能
    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 組織特異性
    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • ドメイン
    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻訳後修飾
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 細胞内局在
    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 別名
    • FADK
    • FADK 1
    • FAK related non kinase polypeptide
    • FAK1
    • FAK1_HUMAN
    • Focal adhesion Kinase
    • Focal adhesion kinase 1
    • Focal adhesion kinase isoform FAK Del33
    • Focal adhesion kinase related nonkinase
    • FRNK
    • p125FAK
    • pp125FAK
    • PPP1R71
    • Protein phosphatase 1 regulatory subunit 71
    • Protein tyrosine kinase 2
    • Protein-tyrosine kinase 2
    • Ptk2
    • PTK2 protein tyrosine kinase 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab215072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

画像

  • Standard calibration curve. Background subtracted values are graphed.

プロトコール

参考文献

ab215072 has not yet been referenced specifically in any publications.

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