製品の概要

  • 製品名Mouse Eotaxin ELISA Kit
    Eotaxin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1 pg/ml
  • 検出範囲
    1.02 pg/ml - 100 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.25 83% - 104%
    Serum 94.95 83% - 104%
    Plasma 92.54 84% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Eotaxin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Eotaxin coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 11
    • CCL 11
    • CCL11
    • CCL11_HUMAN
    • Chemokine (C-C motif) ligand 11
    • Chemokine C C motif ligand 11
    • Chemokine CC Motif Ligand 11
    • Chemokine ligand 11
    • Eosinophil Chemotactic P
    • Eosinophil chemotactic protein
    • Eotaxin
    • Eotaxin 1
    • MGC22554
    • SCYA 11
    • SCYA11
    • Small inducible cytokine A11
    • Small inducible cytokine subfamily A (Cys Cys) member 11
    • Small inducible cytokine subfamily A member 11
    • Small-inducible cytokine A11
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Eotaxin ELISA Kit 画像

  • Representative standard curve using ab100680

  • Representative standard curve using ab100680

プロトコール

Mouse Eotaxin ELISA Kit (ab100680) 使用論文

This product has been referenced in:
  • Ren X  et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). Read more (PubMed: 23149934) »
  • Ci X  et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). Read more (PubMed: 22563373) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"