製品の概要

  • 製品名Mouse DKK1 ELISA Kit (Dickkopf-1)
    DKK1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 25 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 79.55 71% - 85%
    Serum 100.8 91% - 107%
    Plasma 91.38 83% - 100%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s DKK1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in serum (mouse DKK1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  • 別名
    • Dickkopf 1
    • Dickkopf homolog 1
    • Dickkopf related protein 1
    • DKK 1
    • hDkk 1
    • hDkk1
    • mDkk1
    • SK
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse DKK1 ELISA Kit (Dickkopf-1) 画像

  • Representative standard curve using ab113347

  • Representative standard curve using ab113347

プロトコール

Mouse DKK1 ELISA Kit (Dickkopf-1) (ab113347) 使用論文

ab113347 has not yet been referenced specifically in any publications.

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