Mouse CTLA 4 ELISA Kit (ab113346)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.92 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse CTLA 4 ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5 pg/ml -
検出範囲
4.92 pg/ml - 1200 pg/ml -
添加回収試験
96 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 76.34 67% - 89% Serum 107.3 101% - 114% Plasma 104.2 100% - 110% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s CTLA4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTLA4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse CTLA4 coated on a 96- well plate. Standards and samples are pipetted into the wells and CTLA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CTLA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CTLA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CTLA4 2 vials CTLA4 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CTLA4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113346 は論文での使用が確認できていません。