製品の概要

  • 製品名Mouse CD62P ELISA Kit (P-Selectin)
    CD62P キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.62 83% - 105%
    Serum 92.38 80% - 102%
    Plasma 93.57 81% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s P-Selectin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse P-Selectin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse P-Selectin coated on a 96-well plate. Standards and samples are pipetted into the wells and P-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse P-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • 組織特異性Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • 関連疾患Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • 配列類似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • Antigen CD62
    • CD62
    • CD62 antigen like family member P
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP 140
    • GMP-140
    • GMP140
    • Granule membrane protein 140
    • Granule membrane protein 140kDa
    • Granulocyte membrane protein
    • GRMP
    • LECAM3
    • Leukocyte endothelial cell adhesion molecule 3
    • Leukocyte-endothelial cell adhesion molecule 3
    • LYAM3_HUMAN
    • P selectin
    • P-selectin
    • PADGEM
    • Platelet activation dependent granule external membrane protein
    • Platelet activation dependent granule-external membrane protein
    • Platelet alpha granule membrane protein
    • PSEL
    • Selectin P
    • Selectin P (granule membrane protein 140kDa antigen CD62)
    • SELP
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse CD62P ELISA Kit (P-Selectin) 画像

  • Representative standard curve using ab100737

  • Representative standard curve using ab100737

プロトコール

Mouse CD62P ELISA Kit (P-Selectin) (ab100737) 使用論文

ab100737 has not yet been referenced specifically in any publications.

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