製品の概要

  • 製品名Mouse CD153 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.5 pg/ml
  • 検出範囲
    0.41 pg/ml - 100 pg/ml
  • 添加回収試験

    99 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.34 91% - 110%
    Serum 96.78 87% - 108%
    Plasma 95.79 85% - 107%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s CD153 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD153 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CD153 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD153 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD153 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD153 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性CD153, also known as (CD30 ligand), is a 40KD type II transmembrane protein belonging to the TNF superfamily. In mouse CD153 is expressed primarily on activated CD4+ve T cells. The interaction of CD153 with its ligand CD30 is reported to enhance the proliferation of anti CD3/CD28 costimulated T cells.
  • 別名
    • CD153 antigen
    • CD30 antigen ligand
    • CD30 L
    • CD30 ligand
    • CD30L
    • CD30LG
    • MGC138144
    • TNFSF8
    • Tumor necrosis factor (ligand) superfamily member 8
    • Tumor necrosis factor ligand superfamily member 8
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse CD153 ELISA Kit 画像

  • Representative Standard Curve using ab100672

  • Representative Standard Curve using ab100672

     

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Mouse CD153 ELISA Kit (ab100672) 使用論文

ab100672 has not yet been referenced specifically in any publications.

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