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|Cell culture supernatant||95.35||83% - 106%|
|Serum||94.36||82% - 103%|
|Plasma||92.68||80% - 102%|
Abcam’s CCL27 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL27 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CCL27 coated on a 96- well plate. Standards and samples are pipetted into the wells and CCL27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CCL27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|内容||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|700X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Mouse CCL27||2 vials|
|CCL27 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse CCL27 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100676 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100676 has not yet been referenced specifically in any publications.