Mouse Cathepsin B ELISA Kit (ab119585)

製品の概要

  • 製品名
    Mouse Cathepsin B ELISA Kit
    Cathepsin B キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Tissue Extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 5 pg/well
  • 検出範囲
    156 pg/well - 10000 pg/well
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 製品の概要

    Abcam’s Mouse Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin B specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cathepsin B amount of sample captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 機能
    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • 配列類似性
    Belongs to the peptidase C1 family.
  • 細胞内局在
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 別名
    • Amyloid precursor protein secretase
    • APP secretase
    • APPS
    • CATB_HUMAN
    • Cathepsin B heavy chain
    • Cathepsin B1
    • CathepsinB
    • CPSB
    • CTSB
    • cysteine protease
    • OTTHUMP00000116009
    • OTTHUMP00000229510
    • OTTHUMP00000229511
    • OTTHUMP00000229512
    • OTTHUMP00000229514
    • OTTHUMP00000229515
    • OTTHUMP00000229516
    • Preprocathepsin B
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Cathepsin B ELISA Kit 画像

  • Representative Standard Curve Using ab119585.

プロトコール

Mouse Cathepsin B ELISA Kit (ab119585) 使用論文

ab119585 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
I was interested in detecting secreted cathepsin B in rat hippocampal tissue. Although the kit uses antibodies designed against mouse, mouse and rat cathepsin B share >90% homology. I was able to successfully detect cathepsin B in the supernatant from organotypic hippocampal slices (D.I.V. 10-14) cultured from Wistar rats (P7). The assay was sensitive, showing a positive signal when tested with 200uL of serum-free media, which was washed over the slice for 10 minutes prior to processing. The assay also showed low well-to-well variability when identical samples were run on multiple wells. Serum-free culture media showed little or no reactivity.
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Mr. Zahid Padamsey

Verified customer

投稿 Aug 11 2015

Thank you for contacting Abcam.

I do apologize for the delay in returning this information to you, especially as I image you have already found the results from the mistaken mixing ofthe biotinylation solution with sample buffer instead of a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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