Mouse brain tissue lysate - total protein (7 days) (ab7190)

製品の概要

  • 製品名Mouse brain tissue lysate - total protein (7 days)
    Brain ライゼート 製品一覧
  • 製品の詳細
    Mouse brain total protein lysate
  • 特記事項

    Source: Outbred NIH-Swiss Albino mice.

    The mouse brain tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab7190 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent dilution. Mouse brain tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

    Mouse brain tissue lysate - total protein (7 days) (ab7190) 使用論文

    ab7190 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I presume you are looking for positive control used in western blot. Our customers have used this ab successfully with mouse brain tissue and 293 cells lysates. Please check the Abreviews. The Mouse brain lysates ca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"