製品の概要

  • 製品名Mouse BMP2 ELISA Kit
    BMP2 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    62.5 pg/ml - 4000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse BMP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BMP2 in bone tissue, cell culture supernatants and serum.

    A BMP2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BMP2 amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Induces cartilage and bone formation.
  • 組織特異性Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
  • 配列類似性Belongs to the TGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • BDA2
    • BMP-2
    • BMP-2A
    • Bmp2
    • BMP2_HUMAN
    • BMP2A
    • Bone morphogenetic protein 2
    • Bone morphogenetic protein 2A
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse BMP2 ELISA Kit 画像

  • Representative Standard Curve using ab119582.

プロトコール

Mouse BMP2 ELISA Kit (ab119582) 使用論文

This product has been referenced in:
  • Mai Z  et al. Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin ß1 and BMP2 signaling cross-talk. PLoS One 8:e61600 (2013). Read more (PubMed: 23593489) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"