製品の概要

  • 製品名Mouse BCA1 ELISA Kit (BLC)
    BCA1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    1.37 pg/ml - 1000 pg/ml
  • 添加回収試験

    99 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 104.56 90% - 110%
    Serum 96.89 83% - 103%
    Plasma 97.65 84% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s BCA1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BCA1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.
  • 別名
    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell chemoattractant
    • B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)
    • B lymphocyte chemoattractant
    • BCA 1
    • BLC
    • BLR1L
    • C X C motif chemokine 13
    • Chemokine (C X C motif) ligand 13
    • Chemokine (C X C motif) ligand 13 (B cell chemoattractant)
    • Chemokine CXC motif ligand 13
    • CXC chemokine BLC
    • CXCL13
    • SCYB13
    • Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab100671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse BCA1 ELISA Kit (BLC) 画像

  • Representative standard curve using ab100671

  • Representative standard curve using ab100671

プロトコール

Mouse BCA1 ELISA Kit (BLC) (ab100671) 使用論文

ab100671 has not yet been referenced specifically in any publications.

Product Wall



The capture antibody in this sandwich ELISA is a rat monoclonal.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"