製品の概要

  • 製品名
    Mouse BAFF ELISA Kit (TNFSF13B)
    BAFF キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    46.9 pg/ml - 3000 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A BAFF (TNFSF13B) specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BAFF (TNFSF13B) amount of sample captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-mouse BAFF antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- mouse BAFF antibody 1 x 130µl
    Lyophilized recombinant mouse BAFF standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • 研究分野
  • 機能
    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
    Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
  • 組織特異性
    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾
    The soluble form derives from the membrane form by proteolytic processing.
    Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
    N-glycosylated.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • B lymphocyte stimulator
    • B-cell-activating factor
    • BAFF
    • BLyS
    • CD257
    • Dendritic cell-derived TNF-like molecule
    • soluble form
    • TALL-1
    • TN13B_HUMAN
    • TNF- and APOL-related leukocyte expressed ligand 1
    • TNFSF13B
    • Tumor necrosis factor ligand superfamily member 13b
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119580.

プロトコール

参考文献

ab119580 has not yet been referenced specifically in any publications.

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