製品の概要

  • 製品名Mouse B7-1 ELISA Kit (CD80)
    CD80 キット 製品一覧
  • 検出方法Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    62.5 pg/ml - 4000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse CTLA4 (CD80) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse CTLA4 in cell culture supernatants and serum.

    A CTLA4 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for CTLA4 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse CTLA4 amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • 組織特異性Expressed on activated B-cells, macrophages and dendritic cells.
  • 配列類似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • Activation B7-1 antigen
    • B7
    • BB1
    • CD80
    • CD80_HUMAN
    • CTLA-4 counter-receptor B7.1
    • T-lymphocyte activation antigen CD80
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse B7-1 ELISA Kit (CD80) 画像

  • Representative Standard Curve Using ab119578.

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Mouse B7-1 ELISA Kit (CD80) (ab119578) 使用論文

ab119578 has not yet been referenced specifically in any publications.

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