Mouse Antithrombin III ELISA Kit (SERPINC1) (ab108800)


  • 製品名Mouse Antithrombin III ELISA Kit (SERPINC1)
    Antithrombin III キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 4.4%
    サンプル N 平均値 SD CV%
    Overall 9.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 3 ng/ml
  • 検出範囲
    3.13 ng/ml - 100 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.

    An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 組織特異性Found in plasma.
  • 関連疾患Antithrombin III deficiency
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Phosphorylation sites are present in the extracellular medium.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 別名
    • ANT3_HUMAN
    • Antithrombin-III
    • ATIII
    • Serpin C1
    • SERPINC1
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab108800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Antithrombin III ELISA Kit (SERPINC1) 画像

  • Standard curve : mean of duplicates (+/-SD) with backgrounds readings subtracted

  • Ms antithrombine III measured in biological fluids showing quantity (ng) per mL of sample tested


Mouse Antithrombin III ELISA Kit (SERPINC1) (ab108800) 使用論文

ab108800 has not yet been referenced specifically in any publications.

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Thank you for your phone call.
The standards for these kits are supplied in different masses. The mass for each vial will be printed on the label, and each lot will require a different volume of diluent in order to generate the 100 ng/mL standard....

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Thank you for your patience.

Yes, I can confirm that the vial you received is 50x which is correct in this case - for the lot you received. We are looking into updating the datasheet. Going forward 80x will be the correct amount.


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Thank you for contacting us.

I have contacted the corresponding department regarding your inquiry and hope to have an answer for you soon. I will be in touch.

I am sorryfor the confusion, and thank you for your patience.

Thank you for contacting us. I have had a chance to speak with the lab regarding your question. Our data suggested that mouse serum ATIII has slightly higher concentration than plasma. We have about 200 – 600 ug/ml range of different mouse samples....

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Thank you for contacting us. I was hoping that you might fill me in on a couple of points about your results with this assay. Have you been able to plot the standard curve accurately using the mean value of the triplicate readings for each stan...

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