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ab139697 is for simultaneous detection of 24 Mouse Angiogenic factors. Suitable for all sample types.
Targets: Eotaxin, Fas Ligand, bFGF, G-CSF, GM-CSF, IFN-gamma, IGF-II, IL-1 alpha, IL-1 beta, IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, MCP-1, M-CSF, MIG, PF-4, TIMP-1, TIMP-2, TNF-alpha, Thrombopoietin.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
|内容||1 x 4 Membranes||1 x 8 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl|
|1X Blocking Buffer||1 x 25ml||1 x 50ml|
|20X Wash Buffer I||1 x 10ml||1 x 20ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 20ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit|
|Angiogenesis Antibody Array Membranes||4 units||8 units|
|Biotin-Conjugated Anti-Cytokines||2 vials||4 vials|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml|
Our Abpromise guarantee covers the use of ab139697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"