Mouse Angiogenesis 抗体 Array - Membrane (24 Targets) (ab139697)

製品の概要

  • 製品名
    Mouse Angiogenesis Antibody Array - Membrane (24 Targets)
    Angiogenesis Antibody Array antibody arrays 製品一覧
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • アッセイタイプ
    Semi-quantitative
  • 種交差性
    交差種: Mouse
  • 製品の概要

    ab139697 is for simultaneous detection of 24 Mouse Angiogenic factors. Suitable for all sample types.



    Targets: Eotaxin, Fas Ligand, bFGF, G-CSF, GM-CSF, IFN-gamma, IGF-II, IL-1 alpha, IL-1 beta, IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, MCP-1, M-CSF, MIG, PF-4, TIMP-1, TIMP-2, TNF-alpha, Thrombopoietin.


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.



    Learn more about membrane antibody arrays

  • 特記事項

    A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • アプリケーション
    適用あり: Multiplex Protein Detectionmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab139697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Multiplex Protein Detection Use at an assay dependent concentration.

画像

  • Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

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参考文献

This product has been referenced in:

See all 3 Publications for this product

レビューと Q&A

Stripping the membranes and re-probing is not recommended as stripping buffers contain denaturing reagents which can damage the printed array antibodies, especially the positive control spots. As a result, weakened signals may occur. It may be possible...

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Thank you for contacting us. These antibodies are not sold individually, only in the array kit.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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