Mouse alpha Macroglobulin ELISA Kit (ab108887)

製品の概要

  • 製品名Mouse alpha Macroglobulin ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.07 ng/ml
  • 検出範囲
    0.078 ng/ml - 20 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s alpha Macroglobulin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Macroglobulin levels in plasma, serum, urine, and cell culture supernatants.

    An alpha Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Macroglobulin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Mouse alpha Macroglobulin ELISA Kit 画像

  • Typical standard curve using ab108887.

プロトコール

Mouse alpha Macroglobulin ELISA Kit (ab108887) 使用論文

ab108887 has not yet been referenced specifically in any publications.

Product Wall

The locations of the epitopes that the antibodies in the alpha macroglobulin ELISA recognize are unknown. In other words, they have not been mapped.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"