Mouse alpha Macroglobulin ELISA Kit (ab108887)
Key features and details
- Sensitivity: 0.28 ng/ml
- Range: 1 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse alpha Macroglobulin ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.8% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.28 ng/ml -
検出範囲
1 ng/ml - 25 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s alpha Macroglobulin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Macroglobulin levels in plasma, serum, urine, and cell culture supernatants.
An alpha Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Macroglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated alpha Macroglobulin Antibody 1 x 120µl alpha Macroglobulin Microplate (12 x 8 well strips) 1 unit alpha Macroglobulin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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参照データベース
- SwissProt: Q61838 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108887 は論文での使用が確認できていません。