製品の概要

  • 製品名
    Mouse Albumin ELISA Kit
    Albumin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 2.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.9%
  • サンプルの種類
    Cell culture supernatant, Urine
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.7 ng/ml
  • 検出範囲
    1.56 ng/ml - 400 ng/ml
  • 添加回収試験

    97 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Albumin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.


    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve Using ab108792.

プロトコール

参考文献

This product has been referenced in:
  • Chakraborty K  et al. The mito-DAMP cardiolipin blocks IL-10 production causing persistent inflammation during bacterial pneumonia. Nat Commun 8:13944 (2017). Read more (PubMed: 28074841) »
  • Xie Y  et al. Anti-diabetic effect of Alpinia oxyphylla extract on 57BL/KsJ db-/db- mice. Exp Ther Med 13:1321-1328 (2017). Mouse . Read more (PubMed: 28413472) »

See all 9 Publications for this product

レビューと Q&A


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCyto...

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Thank you for your enquiry. I am sorry to hear the customer has some concerns regarding this kit, I hope I can be of help. For the standard,  as described in the protocol this needs to be reconstituted with the appropriate amount of diluent to gene...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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