製品の概要

  • 製品名Mouse Albumin ELISA Kit
    Albumin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma, Tissue Lysate
  • アッセイタイプCompetitive
  • 検出感度
    = 0.09 µg/ml
  • 検出範囲
    0.098 µg/ml - 25 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    2h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 製品の概要

    Abcam’s Albumin Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse Albumin levels in plasma and serum.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 組織特異性Plasma.
  • 関連疾患Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 配列類似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻訳後修飾Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

Mouse Albumin ELISA Kit 画像

  • Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108791.

プロトコール

Mouse Albumin ELISA Kit (ab108791) 使用論文

ab108791 has not yet been referenced specifically in any publications.

Product Wall

This ELISA kit was originally optimized for serum and plasma but our lab recently validated it for use with lysates. This is why there is not a lysis buffer included with the kit but you can use RIPA buffer.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"