製品の概要

  • 製品名
    Mouse Albumin ELISA Kit
    Albumin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • サンプルの種類
    Serum, Plasma, Tissue Lysate
  • アッセイタイプ
    Competitive
  • 検出感度
    = 0.09 µg/ml
  • 検出範囲
    0.098 µg/ml - 25 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    2h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 製品の概要

    Abcam’s Albumin Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse Albumin levels in plasma and serum.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

画像

  • Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108791.

プロトコール

参考文献

ab108791 has not yet been referenced specifically in any publications.

レビューと Q&A

This ELISA kit was originally optimized for serum and plasma but our lab recently validated it for use with lysates. This is why there is not a lysis buffer included with the kit but you can use RIPA buffer.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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