Anti-mouse IgG for IP (Biotin) (ab131367)
Key features and details
- Anti-mouse IgG for IP (Biotin)
- Conjugation: Biotin
- Host species: Rat
- Isotype: IgG
- Suitable for: WB
製品の概要
-
製品名
Anti-mouse IgG for IP (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Rat -
ターゲット生物種
Mouse -
特異性
VeriBlot for IP secondary antibodies are anti-mouse immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa). In general this interference tends to originate from secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself. VeriBlot for IP secondary antibodies only recognize native (non-reduced) antibodies and therefore the detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced. -
アプリケーション
適用あり: WBmore details -
標識
Biotin
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride -
Concentration information loading...
-
ポリ/モノ
モノクローナル -
クローン名
eB144 -
アイソタイプ
IgG -
特記事項
Number of blots:
At least 20 blots (based on a 0.5 µg/ml dilution in 5 ml milk).
Important protocol notes:
1. The anti-mouse VeriBlot for IP secondary antibody (Biotin) detects mouse IgG antibodies (subtypes IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse VeriBlot for IP secondary antibody (Biotin) preferentially detects the non-reduced form of mouse IgG (subtypes: IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-deantured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
5. Anti-mouse IgG VeriBlot for IP secondary antibody (Biotin) (ab131367) is the biotinylated form of the VeriBlot for IP anti-mouse HRP (ab131368). It can be used as a secondary antibody in combination with avidin/streptavidin HRP (ab59653/ab7403).
Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips -
研究分野
関連製品
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131367の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.5 µg/ml. The dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP.
|
特記事項 |
---|
WB
Use a concentration of 0.5 µg/ml. The dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP. |
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab131367 は 1 報の論文で使用されています。
- Yu Z et al. Translocation of gasdermin D induced mitochondrial injury and mitophagy mediated quality control in lipopolysaccharide related cardiomyocyte injury. Clin Transl Med 12:e1002 (2022). PubMed: 36030524