製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to MORC3
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human protein

  • ポジティブ・コントロール
    • Hela cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 107 kDa.

ターゲット情報

  • 機能
    Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism (PubMed:20501696). Sumoylated MORC3-NBs can also associate with PML-NBs (PubMed:20501696). Recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity (PubMed:17332504). Binds RNA in vitro (PubMed:11927593). May be required for influenza A transcription during viral infection (PubMed:26202233).
  • 組織特異性
    Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney.
  • 配列類似性
    Contains 1 CW-type zinc finger.
  • 翻訳後修飾
    Sumoylation is involved in interaction with PML and localization to PML nuclear bodies.
  • 細胞内局在
    Nucleus, nucleoplasm. Nucleus matrix. Nucleus, PML body. Also found in PML-independent nuclear bodies. Localization to nuclear bodies is ATP-dependent.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0136 antibody
    • Microrchidia 3 antibody
    • MORC family CW type zinc finger 3 antibody
    • MORC family CW type zinc finger protein 3 antibody
    • MORC family CW-type zinc finger protein 3 antibody
    • MORC3 antibody
    • MORC3_HUMAN antibody
    • Nuclear matrix protein 2 antibody
    • Nuclear matrix protein NXP2 antibody
    • NXP2 antibody
    • ZCW5 antibody
    • ZCWCC3 antibody
    • Zinc finger CW type coiled coil domain 3 antibody
    • Zinc finger CW type coiled coil domain protein 3 antibody
    • Zinc finger CW type with coiled coil domain 3 antibody
    • Zinc finger CW-type coiled-coil domain protein 3 antibody
    see all

画像

  • Anti-MORC3 antibody (ab76813) at 1/500 dilution + nuclear extracts from Hela cells at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 107 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MORC3 antibody (ab76813) at 1/500 dilution

    Lane 1 : MORC3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 107 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Ver LS  et al. The Cellular Factor NXP2/MORC3 Is a Positive Regulator of Influenza Virus Multiplication. J Virol 89:10023-30 (2015). Read more (PubMed: 26202233) »
  • Ichimura Y  et al. Anti-NXP2 autoantibodies in adult patients with idiopathic inflammatory myopathies: possible association with malignancy. Ann Rheum Dis : (2012). WB ; Human . Read more (PubMed: 22258483) »

See all 2 Publications for this product

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