Anti-Monoacylglycerol Lipase 抗体 (ab77398)

製品の概要

  • 製品名
    Anti-Monoacylglycerol Lipase antibody
    Monoacylglycerol Lipase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Monoacylglycerol Lipase
  • 特異性
    abb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1).
  • アプリケーション
    適用あり: ELISA, IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Synthetic peptide:

    C-QDLPHLVNADGQY

    , corresponding to internal sequence amino acids 17-29 of Human Monoacylglycerol Lipase (NP_009214.1; NP_001003794.1).

  • ポジティブ・コントロール
    • Human Adipose and Brain (Frontal Cortex) lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
IHC-P
WB
IHC-Fr
  • 追加情報
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
    • 組織特異性
      Detected in adipose tissue, lung, liver, kidney, brain and heart.
    • パスウェイ
      Glycerolipid metabolism; triacylglycerol degradation.
    • 配列類似性
      Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 3.1.1.23 antibody
      • HU K5 antibody
      • HU-K5 antibody
      • HUK5 antibody
      • Lysophospholipase homolog antibody
      • Lysophospholipase like antibody
      • Lysophospholipase-like antibody
      • MAGL antibody
      • MGL antibody
      • MGLL antibody
      • MGLL_HUMAN antibody
      • Monoacylglycerol lipase antibody
      • Monoglyceride lipase antibody
      see all

    画像

    • Anti-Monoacylglycerol Lipase antibody (ab77398) at 0.5 µg/ml + Human Adipose lysate (in RIPA buffer) at 35 µg

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • Immunohistochemical staining of Monoacylglycerol Lipase using ab77398 on Formaldehyde-fixed paraffin-embedded mouse adipose tissue sections (from around the kidney).  Antigen retrieval step: heat mediated in citric acid HIER. Blocking step: 1% BSA for 10 mins @ rt°C. ab77398 incubated at 1/500 for 2 hours. Secondary Antibody:  biotin labelled anti goat IgG used at 1/200. The submitted image shows white fat deposits around the kidney. Dilution factors vary between 1/300-500. There is a pale positivity in the renal tubular cytoplasm which appears to be specific (at lower dilution factor this is more obvious but, in this block the fat was too strongly immunostained). Section was endogenous biotin-blocked prior to incubation with primary Ab.

      See Abreview

    参考文献

    ab77398 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Brown adipose tissue)
    Specification
    Brown adipose tissue
    Permeabilization
    Yes - 0.2 % Triton
    Fixative
    Formaldehyde
    Username

    Mrs. Noemi Pasquarelli

    Verified customer

    投稿 Nov 11 2013




    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab77398 in IHC-Fr zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einset...

    Read More

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab77398 Anti-Monoacylglycerol Lipase antibody bisher noch nicht in IHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney adipose tissue)
    Specification
    kidney adipose tissue
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid HIER
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Dec 21 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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