製品の概要
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製品名Anti-Monoacylglycerol Lipase antibody
Monoacylglycerol Lipase 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Monoacylglycerol Lipase
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由来種Goat
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特異性abb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1).
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アプリケーション適用あり: ELISA, IHC-P, WB, IHC-Frmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
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免疫原
Synthetic peptide:
C-QDLPHLVNADGQY
, corresponding to internal sequence amino acids 17-29 of Human Monoacylglycerol Lipase (NP_009214.1; NP_001003794.1). -
ポジティブ･コントロール
- Human Adipose and Brain (Frontal Cortex) lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab77398 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab77398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | ||
| IHC-P | ||
| WB | ||
| IHC-Fr |
追加情報
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
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組織特異性Detected in adipose tissue, lung, liver, kidney, brain and heart.
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パスウェイGlycerolipid metabolism; triacylglycerol degradation.
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配列類似性Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
- Information by UniProt
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参照データベース
- Entrez Gene: 11343 Human
- Entrez Gene: 23945 Mouse
- Entrez Gene: 29254 Rat
- Omim: 609699 Human
- SwissProt: Q99685 Human
- SwissProt: O35678 Mouse
- SwissProt: Q8R431 Rat
- Unigene: 277035 Human
see all -
別名
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
画像
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Western blot - Monoacylglycerol Lipase antibody (ab77398)Anti-Monoacylglycerol Lipase antibody (ab77398) at 0.5 µg/ml + Human Adipose lysate (in RIPA buffer) at 35 µg
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase antibody (ab77398)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemical staining of Monoacylglycerol Lipase using ab77398 on Formaldehyde-fixed paraffin-embedded mouse adipose tissue sections (from around the kidney). Antigen retrieval step: heat mediated in citric acid HIER. Blocking step: 1% BSA for 10 mins @ rt°C. ab77398 incubated at 1/500 for 2 hours. Secondary Antibody: biotin labelled anti goat IgG used at 1/200. The submitted image shows white fat deposits around the kidney. Dilution factors vary between 1/300-500. There is a pale positivity in the renal tubular cytoplasm which appears to be specific (at lower dilution factor this is more obvious but, in this block the fat was too strongly immunostained). Section was endogenous biotin-blocked prior to incubation with primary Ab.
参考文献
ab77398 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"