Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (fibroblast)
Specification
fibroblast
Total protein in input
1000 µg
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Western Blot antibody
Name
Non-Abcam antibody was used: rabbit-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Additional data
Additional Notes
IP was performed with anti-methyl-Arg antibody and western blotting was performed with anti-protein X which is a Arg methylated protein.
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投稿 Jun 19 2014