製品の概要

  • 製品名Monkey Soluble TNF Receptor I ELISA Kit
    TNF Receptor I キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4 2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 4 9%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.06 U/ml
  • 検出範囲
    0.09 U/ml - 6 U/ml
  • 添加回収試験

    93 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Monkey
  • 製品の概要

    Abcam’s Soluble TNF Receptor I Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey Soluble TNF Receptor I concentrations in Cell culture supernatant and serum (baboon, cynomolgus).

    Soluble TNF Receptor I specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated Soluble TNF Receptor I detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is then added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the Soluble TNF Receptor I amount of sample captured in plate.

  • 特記事項

    Standard is recombinant protein produced in E.coli.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • 関連疾患Familial hibernian fever
    Multiple sclerosis 5
  • 配列類似性Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • ドメインThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • 翻訳後修飾The soluble form is produced from the membrane form by proteolytic processing.
  • 細胞内局在Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • 別名
    • CD120a
    • FPF
    • MGC19588
    • p55
    • p55-R
    • p60
    • TBP1
    • TBPI
    • TNF R
    • TNF R55
    • TNF-R1
    • TNF-RI
    • TNFAR
    • TNFR-I
    • TNFR1
    • TNFR55
    • TNFR60
    • TNFRI
    • TNFRSF1a
    • TNR1A_HUMAN
    • Tumor necrosis factor receptor 1
    • Tumor necrosis factor receptor superfamily, member 1A
    • Tumor necrosis factor receptor type 1
    • Tumor necrosis factor receptor type I
    • Tumor necrosis factor-binding protein 1
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab119562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Monkey Soluble TNF Receptor I ELISA Kit 画像

  • Representative Standard Curve using ab119562.

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Monkey Soluble TNF Receptor I ELISA Kit (ab119562) 使用論文

ab119562 has not yet been referenced specifically in any publications.

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