製品の概要

  • 製品名
    Monkey IL2 ELISA Kit (Interleukin-2)
    IL2 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 < 7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8 < 5%
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    10 U/ml
  • 検出範囲
    20 U/ml - 1250 U/ml
  • 添加回収試験

    80 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Monkey
  • 製品の概要

    Abcam’s IL-2 (Interleukin-2) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-2 (Interleukin-2)concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, urine and whole blood.

    IL-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-2 captured on the plate.

  • 特記事項

    Standard is recombinant protein produced in E.coli.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-monkey IL-2 polyclonal antibody 1 x 70µl
    Microplate coated with monoclonal antibody to monkey IL-2 1 unit
    Monkey IL-2 Standard lyophilized (2.4 U/ml upon reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 機能
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 関連疾患
    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 配列類似性
    Belongs to the IL-2 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab119541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119541.

プロトコール

参考文献

ab119541 has not yet been referenced specifically in any publications.

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