Monkey IL-2 ELISA Kit (ab119541)
Key features and details
- Sensitivity: 10 U/ml
- Range: 20 U/ml - 1250 U/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
製品の概要
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製品名
Monkey IL-2 ELISA Kit
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 8 < 7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 8 < 5% -
サンプルの種類
Cell culture supernatant, Serum -
アッセイタイプ
Sandwich (quantitative) -
検出感度
10 U/ml -
検出範囲
20 U/ml - 1250 U/ml -
添加回収試験
80 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Monkey -
製品の概要
Abcam’s IL-2 Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-2 concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, urine and whole blood.
IL-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-2 captured on the plate.
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特記事項
Standard is recombinant protein produced in E.coli.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-monkey IL-2 polyclonal antibody 1 x 70µl Microplate coated with monoclonal antibody to monkey IL-2 1 unit Monkey IL-2 Standard lyophilized 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab119541 は論文での使用が確認できていません。