製品の概要

  • 製品名Monkey IL-6 ELISA Kit (Interleukin-6)
    IL6 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 < 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6 < 10%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    1.3 U/ml
  • 検出範囲
    1.9 U/ml - 120 U/ml
  • 添加回収試験

    90 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Monkey
  • 製品の概要

    Abcam’s IL-6 Monkey (Interleukin-6) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-6 in culture supernatant and serum (baboon, cynomolgus).

    Il-6 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Il-6 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Il-6 captured on the plate.

  • 特記事項

    Standard is recombinant protein produced in E.coli.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 関連疾患Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 配列類似性Belongs to the IL-6 superfamily.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • B cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta-2
    • Interleukin 6
    • Interleukin BSF2
    • Interleukin-6
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab119549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Monkey IL-6 ELISA Kit (Interleukin-6) 画像

  • Representative Standard Curve using ab119549.

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Monkey IL-6 ELISA Kit (Interleukin-6) (ab119549) 使用論文

ab119549 has not yet been referenced specifically in any publications.

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