製品の概要

  • 製品名
    Monkey IL-10 ELISA Kit (Interleukin-10)
    IL10 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 < 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6 < 10%
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    1.3 U/ml
  • 検出範囲
    3.9 U/ml - 250 U/ml
  • 添加回収試験

    95 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Monkey
  • 製品の概要

    Abcam’s IL-10 (Interleukin-10) Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-10 in cell culture supernatant and serum (baboon, cynomolgus).

    This assay employs an antibody specific for Monkey IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Monkey IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Standard is recombinant protein produced in E.coli.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119530.

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参考文献

ab119530 has not yet been referenced specifically in any publications.

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