製品の概要

  • 製品名Monkey E-selectin ELISA Kit
    E-selectin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4 < 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 4 < 10%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.4 U/ml
  • 検出範囲
    1.9 U/ml - 60 U/ml
  • 添加回収試験

    85 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Monkey
  • 製品の概要

    Abcam’s E-selectin monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey E-selectin concentrations in cell culture supernatant and serum.

    E-selectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated E-selectin detection antibody. The microplate is then incubated at room temperature. After the removal of unbound proteins by washing TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of E-selectin captured on the plate.

  • 特記事項

    Standard is recombinant protein produced in E.coli.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 配列類似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD62 antigen-like family member E
    • CD62E
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial adhesion molecule 1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte-endothelial cell adhesion molecule 2
    • LYAM2_HUMAN
    • Sele
    • Selectin E
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab119523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Monkey E-selectin ELISA Kit 画像

  • Representative Standard Curve using ab119523.

プロトコール

Monkey E-selectin ELISA Kit (ab119523) 使用論文

ab119523 has not yet been referenced specifically in any publications.

Product Wall

The product has 120 days guarantee period in Thailand. The 120 days starts form the date of purchase. Expiry date for biological compounds is difficult to guess so we provide guarantee rather than expiry date.
Please store the components as advise...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"