製品の概要

  • 製品名
    Monkey CD62P ELISA Kit
    CD62P キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4 < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 4 < 10%
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    1.3 U/ml
  • 検出範囲
    0.8 U/ml - 50 U/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 76 % - %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Monkey
  • 製品の概要

    Abcam’s CD62P Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey CD62P concentrations in cell culture supernatant, and serum (baboon).

    CD62P specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated CD62P detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the CD62P captured in the plate.

  • 特記事項

    Standard is recombinant protein produced in E.coli.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 2 units
    HRP-Conjugate anti-monkey CD62P monoclonal antibody 1 x 60µl
    Microplate coated with monoclonal antibody to monkey CD62P (12 x 8 wells) 1 unit
    Monkey CD62P Standard Lyophilized (100 U/mL upon reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 機能
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • 組織特異性
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • 関連疾患
    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • 配列類似性
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 別名
    • Antigen CD62
    • CD62
    • CD62 antigen like family member P
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP 140
    • GMP-140
    • GMP140
    • Granule membrane protein 140
    • Granule membrane protein 140kDa
    • Granulocyte membrane protein
    • GRMP
    • LECAM3
    • Leukocyte endothelial cell adhesion molecule 3
    • Leukocyte-endothelial cell adhesion molecule 3
    • LYAM3_HUMAN
    • P selectin
    • P-selectin
    • PADGEM
    • Platelet activation dependent granule external membrane protein
    • Platelet activation dependent granule-external membrane protein
    • Platelet alpha granule membrane protein
    • PSEL
    • Selectin P
    • Selectin P (granule membrane protein 140kDa antigen CD62)
    • SELP
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab119554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119554.

プロトコール

参考文献

ab119554 has not yet been referenced specifically in any publications.

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