製品の概要

  • 製品名
    Anti-MOGT1 antibody
  • 製品の詳細
    Rabbit polyclonal to MOGT1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide designed within C terminal amino acids 288-337 (PIPVRQTLNP TQEQIEELHQ TYMEELRKLF EEHKGKYGIP EHETLVLK) of Human MOGT1 (NP_477513)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Fetal liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

  • 機能
    Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine.
  • パスウェイ
    Glycerolipid metabolism; triacylglycerol biosynthesis.
  • 配列類似性
    Belongs to the diacylglycerol acyltransferase family.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 0610030A14Rik antibody
      • 1110064N14Rik antibody
      • 2-acylglycerol O-acyltransferase 1 antibody
      • Acyl CoA:monoacylglycerol acyltransferase 1 antibody
      • Acyl-CoA:monoacylglycerol acyltransferase 1 antibody
      • DGAT2L antibody
      • DGAT2L1 antibody
      • Diacylglycerol acyltransferase 2 like antibody
      • Diacylglycerol acyltransferase 2-like protein 1 antibody
      • Diacylglycerol O acyltransferase 2 like 1 antibody
      • Diacylglycerol O-acyltransferase candidate 2 antibody
      • hDC2 antibody
      • mDC2 antibody
      • MGAT1 antibody
      • MGC118035 antibody
      • MOGAT1 antibody
      • MOGT1_HUMAN antibody
      • Monoacylglycerol O acyltransferase 1 antibody
      • Monoacylglycerol O-acyltransferase 1 antibody
      • OTTMUSP00000025181 antibody
      see all

    画像

    • Western blot - Anti-MOGT1 antibody (ab81177)
      Western blot - Anti-MOGT1 antibody (ab81177)
      Anti-MOGT1 antibody (ab81177) at 1 µg/ml + fetal liver lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 39 kDa
      Observed band size: 37 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab81177 has not yet been referenced specifically in any publications.

    ab81177 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-MOGT1 antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (stomach)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    60 µg
    Treatment
    RIPA buffer is used for lysis buffer
    Specification
    stomach
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Jul 08 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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