The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Probably involved in connections of major cytoskeletal structures to the plasma membrane.
組織特異性In all tissues and cultured cells studied.
配列類似性Contains 1 FERM domain.
翻訳後修飾Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
細胞内局在Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.