製品の概要

  • 製品名
    Anti-Moesin antibody [38/87]
    Moesin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [38/87] to Moesin
  • 特異性
    ab50007 recognizes the 78 kDa moesin and 80 kDa radixin molecules. It does not react with talin or ezrin.
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-P, Indirect ELISA, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Cow, Human, Pig
  • 免疫原

    Purified bovine uterine moesin

  • ポジティブ・コントロール
    • Jurkat whole cells extract.
  • 特記事項

    The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab151542 as a replacement.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.
  • ポリ/モノ
    モノクローナル
  • クローン名
    38/87
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Indirect ELISA Use at an assay dependent concentration.
Dot blot Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

画像

  • Immunohistochemistry (PFA-fixed) analysis of adult mouse brain tissue labelling Moesin with ab50007. Moesin is shown to be expressed in the adult RMS (rostral migratory stream). Top - low magnification image from DAB staining shows staining along the RMS. Bottom - confocal analysis of doublelabeling with PSA-NCAM shows expression pattern at the cellular level. Moesin was expressed in a number of PSA-NCAM positive cells of the RMS. 

    Brains were sagitally cut on a sliding microtome at 20μm. Immunostaining was preceded by antigen retrieval in sodium citrate, pH 6.0, for 20 min at 98°C followed by 30 minutes cooling at room temperature. For diaminobenzidine (DAB) staining, sections were washed in tris buffered saline (TBS), incubated for 30 minutes in 0.6% H2O2, blocked for 30 minutes with 3% normal donkey serum in 0.1% Triton X-100, and then incubated in primary antibodies for 48 hours at 4°C.

    For fluorescent immunolabeling, free-floating sections were blocked for 30 minutes in 3% normal donkey serum in 0.1% Triton X-100, and then incubated for 48 hours at 4°C in primary antibody. After rinsing in TBS, sections were incubated for 1 hour with biotinylated donkey anti-mouse IgG. Sections were then washed and incubated with Alexa Fluor-conjugated secondary antibodies. ToPro3 was used as a nuclear counterstain.

参考文献

This product has been referenced in:
  • Ji X  et al. Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, ?. Invest Ophthalmol Vis Sci 57:877-88 (2016). Read more (PubMed: 26978024) »
  • Ruppersburg CC & Hartzell HC The Ca2+-activated Cl- channel ANO1/TMEM16A regulates primary ciliogenesis. Mol Biol Cell 25:1793-807 (2014). ICC/IF . Read more (PubMed: 24694595) »

See all 11 Publications for this product

レビューと Q&A

Vielen Dank für Ihre Anfrage.

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Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO)
Loading amount
30 µg
Specification
CHO
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 04 2008

Application
Western blot
Sample
Monkey Cell lysate - whole cell (hepatocyte)
Loading amount
25 µg
Specification
hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (hepatocyte)
Specification
hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepatocyte)
Loading amount
25 µg
Specification
Hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
25 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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