This antibody gave a positive signal in the following Human tissue lysates: Adrenal Gland; Bone Marrow; Brain; Placenta; Skeletal Muscle; Testis; Thymus.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L.
B1, Mps One Binder kinase activator like 1B (yeast) antibody
Chromosome 2 open reading frame 6 antibody
MOB kinase activator 1A antibody
Mob1 alpha antibody
Mob1 homolog 1B antibody
MOB1 Mps One Binder homolog A antibody
MOB1 Mps One Binder kinase activator like 1B antibody
Mob4B protein antibody
Mps one binder kinase activator like 1B antibody
Mps one binder kinase activator-like 1B antibody
Protein Mob4B antibody
Western blot - Anti-MOBK1B antibody (ab110778)
All lanes : Anti-MOBK1B antibody (ab110778) at 1 µg/ml
Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249) Lane 2 : Bone Marrow (Human) Tissue Lysate - adult normal tissue Lane 3 : Human brain tissue lysate - total protein (ab29466) Lane 4 : Human placenta tissue lysate - total protein (ab29745) Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 6 : Human testis tissue lysate - total protein (ab30257) Lane 7 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa Observed band size: 25 kDa Additional bands at: 30 kDa, 44 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110778 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.