製品の概要

  • 製品名Anti-MNK1 (phospho T385) antibody [EPR2370]
    MNK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2370] to MNK1 (phospho T385)
  • 特異性ab109102 recognises MNK1 only when phosphorilated on Threonine 385.
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho synthetic peptide corresponding to residues in the region of T385 of Human MNK1.

  • ポジティブ・コントロール
    • HeLa cell lysates, treated with Calyculin A; Human colon tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
    • 細胞内局在Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • MAP kinase interacting kinase 1 antibody
      • MAP kinase interacting serine/threonine kinase 1 antibody
      • MAP kinase signal integrating kinase 1 antibody
      • MAP kinase signal-integrating kinase 1 antibody
      • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
      • MAPK signal integrating kinase 1 antibody
      • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
      • mknk1 antibody
      • MKNK1_HUMAN antibody
      • MNK 1 antibody
      • Mnk1 antibody
      see all

    Anti-MNK1 (phospho T385) antibody [EPR2370] 画像

    • All lanes : Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 51 kDa
    • Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.

    Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) 使用論文

    This product has been referenced in:
    • Attar-Schneider O  et al. Migration and epithelial-to-mesenchymal transition of lung cancer can be targeted via translation initiation factors eIF4E and eIF4GI. Lab Invest 96:1004-15 (2016). Read more (PubMed: 27501049) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab109102.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"