製品の概要

  • 製品名Anti-MNK1 (phospho T250) antibody
    MNK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MNK1 (phospho T250)
  • 特異性ab111434 detects endogenous levels of MNK1 only when phosphorylated at threonine 250 (Human: Thr250; Mouse: Thr209; Rat: Thr197).
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human MNK1 around the phosphorylation site of threonine 250 (I-T-TP-P-E).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab111434 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • 細胞内局在Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAP kinase interacting kinase 1 antibody
    • MAP kinase interacting serine/threonine kinase 1 antibody
    • MAP kinase signal integrating kinase 1 antibody
    • MAP kinase signal-integrating kinase 1 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
    • MAPK signal integrating kinase 1 antibody
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
    • mknk1 antibody
    • MKNK1_HUMAN antibody
    • MNK 1 antibody
    • Mnk1 antibody
    see all

Anti-MNK1 (phospho T250) antibody 画像

  • ab111434, at 1/50 dilution, staining MNK1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-MNK1 (phospho T250) antibody (ab111434) 使用論文

ab111434 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111434.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"