The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65, 92 kDa.
Use a concentration of 0.1 - 1 µg/ml.
Use at an assay dependent concentration. PubMed: 21791558
機能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
組織特異性Produced by normal alveolar macrophages and granulocytes.
配列類似性Belongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin repeats.
ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻訳後修飾Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
Matrix Metalloproteinase 9 antibody
MMP 9 antibody
Type V collagenase antibody
Anti-MMP9 antibody 画像
Western blot - Anti-MMP9 antibody (ab5707)
PMA stimulated A431 was probed with rabbit anti MMP9 at 1/500 (Lane A). These bands were abolished by blocking peptide (Lane B)
Anti-MMP9 antibody (ab5707) 使用論文
This product has been referenced in:
Sagare AP et al. A lipoprotein receptor cluster IV mutant preferentially binds amyloid-ß and regulates its clearance from the mouse brain. J Biol Chem288:15154-66 (2013).
Read more (PubMed: 23580652) »
Peluffo MC et al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology152:3963-74 (2011).
Read more (PubMed: 21791558) »