製品の概要

  • 製品名Anti-MMP9 antibody [5G3]
    MMP9 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5G3] to MMP9
  • アプリケーション適用あり: WB, ICC, IHC, Flow Cytmore details
  • 種交差性
    交差種: Human, Monkey, Common marmoset
  • 免疫原

    Purified recombinant fragment of Human MMP9 expressed in E. Coli.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in WB with the following lysates: Recombinant protein; MMP9-hIgGFc transfected HEK293 cell lysate; U937; Human tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000.
ICC 1/200 - 1/1000.
IHC 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ターゲット情報

  • 機能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 組織特異性Produced by normal alveolar macrophages and granulocytes.
  • 関連疾患Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody [5G3] 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of common marmoset spleen tissue sections labeling MMP9 with ab119906 at 1/3000 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using citric acid. Tissue was blocked with 1% BSA for 10 minutes at 21°C, followed by incubation with Anti-MMP9 antibody [5G3] (ab119906) in TBS/BSA/azide for 2 hours at 21°C. A biotin conjugated goat anti-mouse IgG secondary antibody was used at 1/200 dilution.

    See Abreview

  • Anti-MMP9 antibody [5G3] (ab119906) at 1/500 dilution + recombinant Human MMP9 protein (238-465 amino acids)Predicted band size : 50.6 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of monkey spleen tissue sections labeling MMP9 with ab119906 at 1/3000 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using citric acid. Tissue was blocked with 1% BSA for 10 minutes at 21°C, followed by incubation with Anti-MMP9 antibody [5G3] (ab119906) in TBS/BSA/azide for 2 hours at 21°C. A biotin conjugated goat anti-mouse IgG secondary antibody was used at 1/200 dilution.

    See Abreview

  • All lanes : Anti-MMP9 antibody [5G3] (ab119906) at 1/10000 dilution

    Lane 1 : Jurkat WCL (negative control)
    Lane 2 : Molt-4 WCL (negative control)
    Lane 3 : U937 WCL (positive control)
    Lane 4 : Human Tonsil (positive control)

    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 82,92 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 seconds
  • All lanes : Anti-MMP9 antibody [5G3] (ab119906) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : MMP7-hIgGFc transfected HEK293 cell lysate
    Lane 3 : MMP9-hIgGFc transfected HEK293 cell lysate
    Predicted band size : 50.6 kDa
  • Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

Anti-MMP9 antibody [5G3] (ab119906) 使用論文

This product has been referenced in:
  • Guo JP & Li XG Galectin-7 promotes the invasiveness of human oral squamous cell carcinoma cells via activation of ERK and JNK signaling. Oncol Lett 13:1919-1924 (2017). WB ; Human . Read more (PubMed: 28454344) »
  • Wang TC  et al. Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12:6435-44 (2015). WB ; Rat . Read more (PubMed: 26299849) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 60 µg
Specification A549
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Apr 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 08 2013

Product Anti-MMP9 antibody [5G3] (ab119906) is provided in a Tissue Culture Supernatant (TCS) format; unfortunately, concentration information for this product is not available.

Antibody concentration is commonly determined by protein assay....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jun 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jun 12 2012

Product Anti-MMP9 antibody [5G3] (ab119906) is generated against a recombinant protein fragment that corresponds to amino acid range aa238-465 of human MMP-9.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"