製品の概要

  • 製品名Anti-MMP8 antibody [MM0023-7A11]
    MMP8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0023-7A11] to MMP8
  • 特異性This antibody recognizes human MMP8 in western blot without cross reactivity with other MMPs.
  • アプリケーション適用あり: IHC-P, Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • Human recombinant MMP8

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml.

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab38994 as Detection.

WB 1/400 - 1/1000. Predicted molecular weight: 53 kDa.

ターゲット情報

  • 機能Can degrade fibrillar type I, II, and III collagens.
  • 組織特異性Neutrophils.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Anti-MMP8 antibody [MM0023-7A11] 画像

  • Standard curve for MMP8; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [MM0023-7A11] to MMP8 (ab56303) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP8 - Hinge region (ab38994) at 0.5µg/ml.
  • ab56303 at 1/200 dilution staining MMP8 in human prostate tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

プロトコール

Anti-MMP8 antibody [MM0023-7A11] (ab56303) 使用論文

This product has been referenced in:
  • Skjøt-Arkil H  et al. Acute Myocardial Infarction and Pulmonary Diseases Result in Two Different Degradation Profiles of Elastin as Quantified by Two Novel ELISAs. PLoS One 8:e60936 (2013). Read more (PubMed: 23805173) »
  • Gabay O  et al. Sirtuin 1 enzymatic activity is required for cartilage homeostasis in vivo in a mouse model. Arthritis Rheum 65:159-66 (2013). IHC ; Mouse . Read more (PubMed: 23124828) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells transfected with human MMP8)
Loading amount 30 µg
Specification HEK293 cells transfected with human MMP8
Treatment cell lysate (TRC), conditioned media (TRM)
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Apr 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"