製品の概要

  • 製品名Anti-MMP8 antibody
    MMP8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MMP8
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MMP8.

    (Peptide available as ab99162.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates:lung; testis; ovary; and in the following whole cell lysates: MOLT4; Jurkat; Raji.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

ターゲット情報

  • 機能Can degrade fibrillar type I, II, and III collagens.
  • 組織特異性Neutrophils.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Anti-MMP8 antibody 画像

  • All lanes : Anti-MMP8 antibody (ab89834) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human ovary tissue lysate - total protein (ab30222)
    Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-MMP8 antibody (ab89834) 使用論文

ab89834 has not yet been referenced specifically in any publications.

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I have not yet heard back from the lab regarding ab81286, but hopefully later today.

However,I checked the homology between human and mousefor the sequence range101-284:it is83%, with poor alignment in ...

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