製品の概要

  • 製品名Anti-MMP7 antibody
    MMP7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MMP7
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Sheep, Horse, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human MMP7.

    (Peptide available as ab95052.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in A431 whole cell lysate and in the following human tissue lysates; Placenta; Liver; Kidney; Small Intestine.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Also detects a band at approximately 22 kDa.

ターゲット情報

  • 機能Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • 配列類似性Belongs to the peptidase M10A family.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Matrilysin antibody
    • Matrin antibody
    • Matrix Metalloproteinase 7 antibody
    • Matrix metalloproteinase-7 antibody
    • MMP 7 antibody
    • MMP-7 antibody
    • MMP7 antibody
    • MMP7_HUMAN antibody
    • MPSL1 antibody
    • PUMP 1 antibody
    • Pump 1 protease antibody
    • Pump-1 protease antibody
    • PUMP1 antibody
    • Uterine matrilysin antibody
    • Uterine metalloproteinase antibody
    see all

Anti-MMP7 antibody 画像

  • All lanes : Anti-MMP7 antibody (ab85144) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)
    Lane 5 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 22,29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • IHC image of MMP7 staining in human pancreatic cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85144, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-MMP7 antibody (ab85144) 使用論文

ab85144 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85144.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"