製品の概要
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製品名Anti-MMP3 antibody [EP1186Y]
MMP3 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP1186Y] to MMP3
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由来種Rabbit
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特異性79% identities with MMP10
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アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP3 aa 450 to the C-terminus (C terminal).
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ポジティブ･コントロール
- Human gastric carcinoma tissue.
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特記事項
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
精製度Protein A purified
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ポリ/モノモノクローナル
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クローン名EP1186Y
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab52915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). | |
| IHC-P | 1/50. | |
| ICC/IF | 1/100 - 1/250. |
ターゲット情報
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機能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
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配列類似性Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 4314 Human
- Entrez Gene: 17392 Mouse
- Entrez Gene: 171045 Rat
- Omim: 185250 Human
- SwissProt: P08254 Human
- SwissProt: P28862 Mouse
- SwissProt: P03957 Rat
- Unigene: 375129 Human
see all -
別名
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase-3 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)](http://a.abcam.com/ps/products/52/ab52915/Images/ab52915-6-ab52915IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (ab52915)](http://a.abcam.com/ps/products/52/ab52915/Images/ab52915-7-ab52915ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)](http://a.abcam.com/ps/products/52/ab52915/Images/ab52915-256958-ab52915WB1.jpg)
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)](http://a.abcam.com/ps/products/52/ab52915/Images/ab52915-5-ab52915IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)](http://a.abcam.com/ps/products/52/ab52915/Images/ab52915-4-ab52915IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
参考文献
This product has been referenced in:
- O'Callaghan J et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). IHC ; Mouse . Read more (PubMed: 28158775) »
- McKay TB et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . Read more (PubMed: 28426715) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"