リコンビナント
RabMAb

Anti-MMP3 抗体 [EP1186Y] (ab52915)

製品の概要

  • 製品名
    Anti-MMP3 antibody [EP1186Y]
    MMP3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1186Y] to MMP3
  • 特異性
    79% identities with MMP10
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP3 aa 450 to the C-terminus (C terminal).

  • ポジティブ・コントロール
    • Human gastric carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-MMP3 antibody (Alexa Fluor® 488) [EP1186Y] (ab194980)

    Anti-MMP3 antibody (Alexa Fluor® 647) [EP1186Y] (ab194717)

    Anti-MMP3 antibody (HRP) [EP1186Y] (ab194716)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

アプリケーション

Our Abpromise guarantee covers the use of ab52915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

Anti-MMP3 antibody [EP1186Y] 画像

  • Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-MMP3 antibody [EP1186Y] (ab52915) 使用論文

This product has been referenced in:
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). IHC ; Mouse . Read more (PubMed: 28158775) »
  • McKay TB  et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . Read more (PubMed: 28426715) »

See all 23 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Alon

Verified customer

投稿 Mar 16 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step
None
Sample
Human Tissue sections (cartilage)
Specification
cartilage
Permeabilization
No
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Mar 19 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (cartilage)
Specification
cartilage
Permeabilization
No
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Mar 19 2015

Thank you for contacting us.



This product will detect total MMP3.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

The MSDS can be downloaded from website. It is now linked to the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
<...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録