アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Synthetic peptide derived from human MMP3 Peptide available as ab134501.
Our Abpromise guarantee covers the use of ab53015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human MMP3 peptide (ab134501).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma labeling MMP3 with ab53015 at 1/100 dilution. Tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using citrate buffer (pH 6.0). The tissue was blocked with 1% serum for 1 hour at 25°C. A biotin conjugated polyclonal goat anti-rabbit secondary antibody was used at 1/200 dilution.
Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"